Which of the following can influence the game's frame rate on various platforms in Unity?

The influence of Quality Settings on a game's frame rate in Unity stems from the way these settings control the graphical fidelity and performance trade-offs. Quality Settings allow developers to adjust the rendering quality for their game, affecting various aspects such as texture resolutions, shadow quality, anti-aliasing, and more. By modifying these parameters, developers can optimize performance for different hardware capabilities, which can significantly impact the game's frame rate.

For instance, higher quality settings that enable advanced graphical effects can lead to a decreased frame rate on lower-end systems, while lowering those settings can help achieve smoother performance. Hence, the Quality Settings provide a critical way to balance visual fidelity and performance across different platforms.

Other aspects like audio settings, lighting settings, and physics calculations can also affect frame rates, but they are generally considered as secondary factors in terms of rendering performance. While they can contribute to performance bottlenecks, Quality Settings offer a more direct and significant method of managing the overall frame rate and is a key feature within Unity that developers frequently adjust to ensure optimal performance across various devices.